Wojciech Szczesny played the whole game as Roma lost 1-0 to Fiorentina.
Takuma Asano made his first start for Stuttgart and played 78 minutes in the 1-0 victory over Kaiserslautern.
Jack Wilshere started for Bournemouth, with the midfielder playing 68 minutes of the 4-0 loss to Manchester City.
Joel Campbell started but was taken off at half-time in a tactical change as Sporting Lisbon lost 3-1 to Rio Ave.
Glen Kamara came on as a substitute in the 34th minute as Colchester United drew 1-1 with Barnet.
Tafari Moore made his senior debut as he was brought on in the 66th minute of Utrecht’s 5-1 defeat to Groningen.
Kelechi Nwakali was introduced as a substitute in stoppage time as he made his first senior appearance for Maastricht in the 1-0 win over Ajax II. Stefan O’Connor was an un-used substitute.
Calum Chambers was an un-used substitute as Middlesbrough lost 3-1 to Everton.
Dan Crowley was an un-used substitute as Oxford United drew 0-0 with MK Dons.
Ryan Huddart was an un-used substitute as Eastleigh beat Macclesfield Town 1-0.
Jon Toral didn’t feature as Granada drew 2-2 with Real Betis.
Julio Pleguezuelo didn’t feature as Mallorca beat Girona 1-0.